Composite

Part:BBa_K2150010:Design

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-06)


This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used RBS BBa_B0033 to reduce leakage, and there is a RiboJ (an insulator element) directly upstream to the CDS in order to reduce the background noise.


Source

This gene is acquired by PCR from genome of Bacillus subtilis

References